While younger and older adults can perform upper-limb reaches to spatial targets with comparable endpoint accuracy (i.e., Helsen et al., 2016; Goodman et al., 2020), movement planning (i.e., reaction time) is significantly longer in older versus younger adults (e.g., Pohl et al., 1996; Goodman et al., 2020). Critically relevant to the current study, age-related differences in reaction time are even greater when older adults plan movement towards somatosensory versus visual or bimodal targets in the absence of vision of the moving limb (e.g., Goodman et al., 2020). One proposed explanation of these lengthened reaction times to somatosensory targets is that older adults may be experiencing challenges in implementing sensorimotor transformations when planning discrete movements of their unseen limb. https://www.selleckchem.com/products/c1632.html To test this idea and assess the contributions of somatosensory information to these motor planning processes, tendon vibration was applied to the muscles of the effector limb between reaching movements made towards visual, somatosensory, or bimodal targets. The results revealed that older adults show the greatest increases in reaction times when vibration was applied during the preparation of movements to somatosensory targets. Further, both older and younger adults exhibited decreased movement endpoint precision when tendon vibration was applied. However, only older adults showed significantly lower movement endpoint precision due to tendon vibration when making movements to somatosensory targets, versus both visual and bimodal targets. These results corroborate previous evidence that older adults have difficulties planning upper-limb movements to somatosensory targets. As well, these results yielded novel evidence that such motor planning processes in older adult rely on somatosensory cues from the effector limb.The Strength, Assistance for walking, Rise from a chair, Climb stairs and Falls (SARC-F) score is a tool recommended for screening the risk of sarcopenia in older patients. However, the association between SARC-F or SARC-F + calf circumference (SARC-F + CC) and the Charlson Comorbidity Index (CCI) in hospitalized older cancer patients is not fully understood. Thus, our aim is to evaluate the association between the SARC-F or SARC-F + CC and the presence of comorbidities and risk of death in older hospitalized cancer patients. A cross-sectional study involving 90 (42 M/48F) hospitalized cancer patients over 60 years old with ongoing chemotherapy or surgical treatment is carried out. The SARC-F is performed to assess the muscle function loss (MFL if SARC-F ? 4), sarcopenia (SARC-F ? 6) and sarcopenia using the calf circumference (SARC-F + CC ?11). CC is assessed using an inelastic tape. The CCI is used to assess the presence of comorbidities. Logistic regression is used to evaluate the association between the Sect comorbidities and risk of death than the use of SARC-F with the calf circumference.A prolonged vasoconstriction/hypoperfusion/hypoxic event follows self-terminating focal seizures. The ketogenic diet (KD) has demonstrated efficacy as a metabolic treatment for intractable epilepsy and other disorders but its effect on local brain oxygen levels is completely unknown. This study investigated the effects of the KD on tissue oxygenation in the hippocampus before and after electrically elicited (kindled) seizures and whether it could protect against a seizure-induced learning impairment. We also examined the effects of the ketone β-hydroxybutyrate (BHB) as a potential underlying mechanism.
Male and female rats were given access to one of three diet protocols 2weeks prior to the initiation of seizures KD, caloric restricted standard chow, and ad libitum standard chow. Dorsal hippocampal oxygen levels were measured prior to initiation of diets as well as before and after a 10-day kindling paradigm. Male rats were then tested on a novel object recognition task to assess postictal learning impairments. In a separate cohort, BHB was administered 30min prior to seizure elicitation to determine whether it influenced oxygen dynamics.
The KD increased dorsal hippocampal oxygen levels, ameliorated postictal hypoxia, and prevented postictal learning impairments. Acute BHB administration did not alter oxygen levels before or after seizures.
The ketogenic diet raised brain oxygen levels and attenuated severe postictal hypoxia likely through a mechanism independent of ketosis and shows promise as a non-pharmacological treatment to prevent the postictal state.
The ketogenic diet raised brain oxygen levels and attenuated severe postictal hypoxia likely through a mechanism independent of ketosis and shows promise as a non-pharmacological treatment to prevent the postictal state.Chemokines have been reported to play important roles in atherosclerotic development. Recently, we found C-C motif ligand 8 (CCL8), a rarely studied chemokine in atherosclerosis, was highly expressed in the endothelium of advanced human carotid plaques. We hypothesized whether CCL8 promotes atherosclerosis through endothelial dysfunction. Apolipoprotein E-deficient mice under the Western diet were used to construct atherosclerosis models. Adeno-associated viruses (AAV) with CCL8 and the CCL8-antibody were injected into mice respectively to conduct CCL8 overexpression and suppression. The results showed that atherosclerotic lesions were significantly increased in the AAV-CCL8 group, while, lesions in the aortic sinus were reduced in the CCL8-antibody group. With CCL8 treatment (200 ng/ml, 24 h) in vitro, the permeability of human aortic endothelial cells (HAECs) increased and the expression of junctional proteins Zonula occluden-1, and Vascular endothelial cadherin were decreased. This effect was dependent on reactive oxygen species (ROS) generation, which could be blocked by l-Ascorbic acid and Apocynin. Results showed that NADPH oxidase 2 (NOX2) expression also increased with CCL8 stimulation and the ROS, and permeability increase of HAECs could be inhibited when NOX2 interfered with the specific siRNA. Additionally, we further found ERK1/2, PI3K-AKT, and NF-κB pathways were involved in the activation of CCL8. Our results indicated that CCL8 might also play important roles in atherosclerosis and this effect, at least in part, was caused by NOX2/ROS-induced endothelial permeability increase. This study might contribute to a deeper understanding of the connection between chemokines and atherosclerosis.